
Results Autophagy regulates Fn14 turnover To identify the mechanism that governs the turnover of Fn14 we first inhibited both lysosomal and ADI 6G degradation in HeLa cells by Bafilomycin A1 BafA and Velcade, respectively Fig. Accordingly, Fn14 also accumulated upon treatment with the well-known acidification inhibitors ammonium chloride NH4Cl or chloroquine CQ 3738 Supplementary Fig. We then followed the subcellular localization of Fn14 by confocal immunofluorescence microscopy. Inhibition of lysosomal degradation resulted in increased ADI 6G of Fn14 to perinuclear ...